An evaluation of the Sysmex NE-8000 hematology analyzer.
نویسندگان
چکیده
The Sysmex NE-8000 is a new, fully automated hematology analyzer capable of providing a five-part white blood cell differential count and identifying abnormal specimens. This instrument was evaluated on 5,000 consecutive blood specimens and compared to the Coulter S Plus-IV analyzer and manual differential cell counts to determine the efficacy of its five-cell differential and screening capabilities. There was a high correlation between the commercial counters for the standard parameters, white blood cell count, red blood cell count, hemoglobin level, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin concentration, and platelet count (r greater than 0.95), except for the mean corpuscular hemoglobin concentration (r = 0.51), for which the NE-8000 was considered the more accurate measurement. Precision and linearity studies were excellent. The white blood cell count, red blood cell count, hemoglobin level, and platelet count were reproducible on specimens stored at 4 degrees C or room temperature for 72 hours and the differential counts were reproducible for 12 hours. The correlations between automated and manual counts for neutrophils, eosinophils, basophils, and lymphocytes were excellent: r = 0.912, 0.945, 0.332, and 0.964, respectively. The monocyte correlation improved with software modification from 0.306 to 0.801. The NE-8000 gave accurate and reproducible differential counts for neutrophils, lymphocytes, and monocytes on specimens with white blood cell counts as low as 0.6 x 10(9)/1. The ability of the instrument to 'flag' abnormal specimens was excellent. The false-positive rate on normal samples was 1.8%, and the false-negative rate on known abnormal samples was 0.3%, due only to nonrecognition of a mild left shift. The identification of specific abnormalities was less precise. The NE-8000 is a powerful hematology analyzer that can perform a five-part white blood cell differential count accurately for a wide range of WBCs and reliably indicate abnormal specimens. It is an excellent screening tool for distinguishing between normal and abnormal specimens and identifying those that require microscopy. Its reliability significantly reduces the need for manual film examination.
منابع مشابه
Evaluation of the Diagnostic Performance of theSysmex XT-2000i Automated Hematology Analyzerin the Detection of Immature Granulocyte
Immature Granulocytes (IG) are normally not present in peripheral blood. Their presence is an important indicator of enhanced bone marrow activation. The present study evaluated the diagnostic performance of the Sysmex XT-2000i automated hematology analyzer in the detection of myelocytes and/or metamyelocytes in patients affected by non-haematological disease, in comparison with manual microsco...
متن کاملPerformance characteristics of the Sysmex XT-2000i hematology analyzer.
The XT-2000i is a new hematology analyzer combining aperture impedance and optical technologies. It is capable of generating a 30-parameter hematologic assay on peripheral blood at a throughput of 80 samples per hour. We have evaluated the performance characteristics of the XT-2000i analyzer and compared them with those of the state-of-the-art XE-2100 analyzer and the manual differential count....
متن کاملDetecting human CD34+ and CD34- hematopoietic stem and progenitor cells using a Sysmex automated hematology analyzer.
In clinical medicine, particularly in the newly developing stem cell therapies required to support the practice of regenerative medicine, the measurement of both CD34+ and CD34- hematopoietic stem cells (HSC)/hematopoietic progenitor cells (HPC) is important in obtaining more accurate information about the total HSC/HPC content in various stem/progenitor cell sources. We report the results of a...
متن کاملPerformance evaluation of the immature granulocyte parameter on the Sysmex XE-2100 automated hematology analyzer.
Recently, St. Anthony's Medical Center Laboratory (SAMC) in St. Louis, Missouri, installed a new automated hematology analyzer, the Sysmex XE-2100. The XE-2100 has an additional differential parameter, the immature granulocyte (IG) parameter. Prior to implementation, the IG parameter was evaluated by comparison to the manual immature granulocyte count. Immature granulocytes counted manually, as...
متن کاملCase Report: Cold Agglutinin Disease; A Laboratory Challenge
Introduction: Autoimmune haemolytic anemia (AIHA) is a complex process characterized by an immune reaction against red blood cell self-antigens. The analysis of specimens, drawn from patients with cold auto-immune hemolytic anemia is a difficult problem for automated hematology analyzer. This paper was written to alert technologists and pathologists to the presence of cold agglutinins and its e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- American journal of clinical pathology
دوره 96 5 شماره
صفحات -
تاریخ انتشار 1991